Home » cDNA Synthesis Protocol by Reverse Transcription cdna technology

cDNA Synthesis Protocol by Reverse Transcription cdna technology

by haisanPHANTHIET



Hey Friends,
cDNA (complementary DNA) is synthesized from an RNA template. This is done by Reverse Transcription. In this example, the oligo d(T)-primers are used to prime the cDNA synthesis. An alternative would be to use random hexamers as primers. cDNA is synthesized before qPCR to check gene expression levels.

qPCR:

References:
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Cheers
Henrik .

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cDNA Synthesis Protocol by Reverse Transcription

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cDNA Synthesis Protocol by Reverse Transcription
cdna technology
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You can see a lot of useful information here: see more here

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30 comments

Nxls yo 14/12/2021 - 8:13 Chiều

deutsch wär echt cooler gewesen

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Fantasy Sehun 14/12/2021 - 8:13 Chiều

i was confused for intron thingy and u made it easy

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Beatriz Vieira 14/12/2021 - 8:13 Chiều

I was so confused with these terms and you explained so clearly and so well. Thank you so much

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MUHAMMAD ANEES 14/12/2021 - 8:13 Chiều

Why cant we use mRNA directly in microarray or qPCR

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Jeff Stoker 14/12/2021 - 8:13 Chiều

Man trying to play God not our place to mess with God's code that makes us human.

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Praveen M 14/12/2021 - 8:13 Chiều

Thank you so much sir❤

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john tindell 14/12/2021 - 8:13 Chiều

Thank you so much. The explanation is very intelligible.

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ahmad omari 14/12/2021 - 8:13 Chiều

amazing , keep going

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It's My Bio 14/12/2021 - 8:13 Chiều

Thank You So much Sir.. I watched lot of Videos But No one explained about RNaseH Function and I was really confused. You Really Explained very nicely. Thanks for making this video..

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debre markos university vine 14/12/2021 - 8:13 Chiều

Thank u so much. before I watch this video I read some what notes but they were boring. ur video were so interesting with smooth sound and full information.

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Sanjana Chaudhary 14/12/2021 - 8:13 Chiều

Thank you soooo much for this.
This thing was more like rocket science to me before watching this video. Cleared all the concepts in just one go.
Thanks again. 😊
Have great day ahead

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Zenyatta The Peaceful 14/12/2021 - 8:13 Chiều

Excellent video. You made this concept easy to understand, thank you.

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ATTILA HORVATH 14/12/2021 - 8:13 Chiều

Wait a second people…forgive my lack of understanding but i dont understand why start to syntettize anything about and HUMAN DNA??? Why want to know what GOD did once and why and how and how to REVERSE IT/////?????!! Please someone explain..Many thanks

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Mario Garrote Filho 14/12/2021 - 8:13 Chiều

Fantastic! Amazing! Incredible! Astounding! Mighty! Wonder! Super!

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Abhinav 14/12/2021 - 8:13 Chiều

Thank you so much Henrik's Lab. You are doing a very noble job by providing us such useful concept clearing video. Through your videos we are able to understand the topic better than hours of classroom learning. ❤❤

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Juana Montoya Venegas 14/12/2021 - 8:13 Chiều

Muy buen video! Gracias!

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Sarah mahmoud 14/12/2021 - 8:13 Chiều

Thank you very much..

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Седа Гаспарян 14/12/2021 - 8:13 Chiều

thank you very very much!

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Gabrielle Haddad 14/12/2021 - 8:13 Chiều

Thanks!!!!

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Vitor Fernando 14/12/2021 - 8:13 Chiều

Amazing! Thanks!

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J DA 14/12/2021 - 8:13 Chiều

This is great. Perfect rythm. Thank you.

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Kevin Jones 14/12/2021 - 8:13 Chiều

Is there a way we could translate all the proteins in a human genome and create a lab created human since the entire genome has been sequenced?

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Li Cheuk Hin 14/12/2021 - 8:13 Chiều

in 2:57 may I ask how whole mrna are replaced by the DNA? thank you

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Sabin Acharya 14/12/2021 - 8:13 Chiều

Hello, i am facing problem in cdna synthesis. would you please help me to know your protocol?

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Flo Sto 14/12/2021 - 8:13 Chiều

Bist du deutscher ? So hörst du dich an XD

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elvis 14/12/2021 - 8:13 Chiều

Sehr verständlich, vielen Dank!

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Sabin Acharya 14/12/2021 - 8:13 Chiều

Thank you. How can we contact you if we get problem while doing qpcr wirk?

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Applewhite 14/12/2021 - 8:13 Chiều

Keep up the good work

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Balti ZonE 14/12/2021 - 8:13 Chiều

Sir
I have lack of word to thanks you.
Really you I m very excited after watched this .
Stay bless forever God bless you More and more forever ❣️💝

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muneeb1050 14/12/2021 - 8:13 Chiều

Thanks 🙂

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